CCL17 ELISA Kit (Monkey)

CAT: 0247-OKCA00868-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA00868-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
C-C motif chemokine 17
Gene Aliases
CC chemokine TARC, C-C motif chemokine 17, Small-inducible cytokine A17, TARC, Thymus and activation-regulated chemokine
Gene ID
574180
Accession Number
NP_001028024.1
Reactivity
Monkey
Target
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CCL17 ELISA Kit (Monkey) (OKCA00868) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CCL17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCL17 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
39 pg/mL-2,500 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%)
Sensitivity
9.8 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CCL17
Protein Name
C-C motif chemokine 17
Gene Name URL
CCL17
Nucleotide Accession Number
NM_001032852.1

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