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CCL17 ELISA Kit (Mouse)

CAT: 0247-OKCA00162-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00162-96WSize:96 Well
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 187.77 201.776 210.341 199.733 181.252 200.108 SD 0.084 0.041 0.083 0.068 0.118 0.125 CV% 6.541 3.025 5.837 5.036 9.478 9.218 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (pg/ml) 187.77 201.776 210.341 199.733 181.252 200.108 Mean (pg/ml) 187.77 201.776 210.341 199.733 181.252 200.108 SD 0.084 0.041 0.083 0.068 0.118 0.125 SD 0.084 0.041 0.083 0.068 0.118 0.125 CV% 6.541 3.025 5.837 5.036 9.478 9.218 CV% 6.541 3.025 5.837 5.036 9.478 9.218
CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 17
Gene Aliases
TA, Scya, Tarc, ABCD-, Abcd-2, Scya17, Scya17l
Gene ID
20295
Accession Number
NP_035462
Reactivity
Mouse
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CCL17 ELISA Kit (Mouse) (OKCA00162) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse CCL17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mouse CCL17 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse CCL17 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell culture supernates and Tissue homogenates
Detection Range
31.25 pg/mL - 2,000 pg/mL
Sensitivity
< 10.512 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TARC. No significant cross-reactivity or interference between mouse TARC and analogues was observed.
Protein Length
103
NCBI Gene Symbol
CCL17
Protein Name
C-C motif chemokine
Gene Name URL
CCL17
Nucleotide Accession Number
XM_006530783