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SCGB3A2 ELISA Kit (Human)

CAT: 0247-OKCA00806-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00806-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Secretoglobin family 3A member 2
Gene Aliases
LU103, PNSP1, UGRP1, pnSP-1
Gene ID
117156
Accession Number
NP_473364.1
Reactivity
Human
Target
The protein encoded by this gene is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology SCGB3A2 ELISA Kit (Human) (OKCA00806) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCGB3A2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCGB3A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SCGB3A2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
46.88 pg/mL-3,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 91 85-99 EDTA Plasma (n=4) 95 89-100 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 91 85-99 Serum (n=5) 91 85-99 EDTA Plasma (n=4) 95 89-100 EDTA Plasma (n=4) 95 89-100
Sensitivity
11.72 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SCGB3A2
Protein Name
Secretoglobin family 3A member 2
Gene Name URL
SCGB3A2
Nucleotide Accession Number
NM_054023.4

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