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Testosterone ELISA Kit (Rat)

CAT: 0247-OKCA00179-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00179-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/mL 1.686 1.566 1.568 1.573 1.585 1.548 SD 0.039 0.046 0.04 0.045 0.051 0.028 CV% 6.435 7.435 6.468 7.288 8.284 4.498 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/mL 1.686 1.566 1.568 1.573 1.585 1.548 Mean (ng/mL Mean (ng/mL 1.686 1.566 1.568 1.573 1.585 1.548 SD 0.039 0.046 0.04 0.045 0.051 0.028 SD 0.039 0.046 0.04 0.045 0.051 0.028 CV% 6.435 7.435 6.468 7.288 8.284 4.498 CV% 6.435 7.435 6.468 7.288 8.284 4.498
CAS Number
7732-18-5
Gene Aliases
Testosterone, Testoderm, Sustanon, Testosteron, Androderm, Mertestate
Reactivity
Rat
Target
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Testosterone ELISA Kit (Rat) (OKCA00179) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Testosterone and Horseradish Peroxidase (HRP) conjugated Testosterone. The competitive inhibition reaction is launched between with HRP labeled Testosterone and unlabeled Testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Testosterone in the sample. The color development is stopped and the intensity of the color is measured.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell culture supernates and Tissue homogenates
Detection Range
0.13 ng/mL - 25.6 ng/mL
Recovery
Sample 1 Sample Type Average Recovery Range Serum (n = 5) 0.9 87-95% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n = 5) 0.9 87-95% Serum (n = 5) 0.9 87-95% Sample 2 Sample Type Average Recovery Range EDTA plasma (n = 4) 0.94 90-98% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA plasma (n = 4) 0.94 90-98% EDTA plasma (n = 4) 0.94 90-98%
Sensitivity
0.06 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Testosterone
Protein Name
Testosterone
Gene Name URL
Testosterone

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