Testosterone ELISA Kit (Mouse)

• Reproducibility:

  Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/mL) 1.618 1.605 1.612 1.617 1.629 1.591 SD 0.041 0.047 0.041 0.046 0.052 0.029 CV% 6.67 7.67 6.703 7.523 8.519 4.733 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/mL) 1.618 1.605 1.612 1.617 1.629 1.591 Mean (ng/mL) 1.618 1.605 1.612 1.617 1.629 1.591 SD 0.041 0.047 0.041 0.046 0.052 0.029 SD 0.041 0.047 0.041 0.046 0.052 0.029 CV% 6.67 7.67 6.703 7.523 8.519 4.733 CV% 6.67 7.67 6.703 7.523 8.519 4.733

• Gene Aliases:

  Testosterone, Testoderm, Sustanon, Testosteron, Androderm, Mertestate

• Reactivity:

  Mouse

• Target:

  Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Testosterone ELISA Kit (Mouse) (OKCA00142) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Testosterone and Horseradish Peroxidase (HRP) conjugated Testosterone. The competitive inhibition reaction is launched between with HRP labeled Testosterone and unlabeled Testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Testosterone in the sample. The color development is stopped and the intensity of the color is measured.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue homogenates

• Detection Range:

  1 - 15 ng/mL

• Sensitivity:

  < 0.05 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  Testosterone

• Protein Name:

  Testosterone

• Gene Name URL:

  Testosterone

• CAS Number:

  7732-18-5