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APOE ELISA Kit (Mouse)

CAT: 0247-OKCA00134-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00134-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay: CV% < 8% (n = 20) . Inter-assay: CV% < 10% (n = 20)
CAS Number
7732-18-5
Gene Name
Apolipoprotein E
Gene Aliases
A, Apo-E, AI255918
Gene ID
11816
Accession Number
NP_001292748
Reactivity
Mouse
Target
This gene encodes a member of the apolipoprotein A1/A4/E family of proteins. This protein is involved in the transport of lipoproteins in the blood. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Homozygous knockout mice for this gene accumulate high levels of cholesterol in the blood and develop atherosclerosis. Different alleles of this gene have been associated with either increased risk or a protective effect for Alzheimer's disease in human patients. This gene maps to chromosome 7 in a cluster with the related apolipoprotein C1, C2 and C4 genes.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology APOE ELISA Kit (Mouse) (OKCA00134) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse APOE and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to APOE and incubated. APOE immobilized in the wells competes with the liquid phase APOE contained in the samples or standards for binding with the HRP-Conjugated APOE Antibody. Wells are washed removing unbound APOE, unbound HRP-Conjugated APOE Antibody or APOE/HRP-Conjugated APOE Antibody complex. HRP-Conjugated APOE Antibody bound to APOE immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated APOE Antibody captured in the well and inversely proportional to the amount of APOE which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernatants, Tissue homogenates
Detection Range
6.25 ng/mL - 400 ng/mL
Recovery
Sample 1 Sample Type Average Recovery Range Serum (n=5) 97% 93-100% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 97% 93-100% Serum (n=5) 97% 93-100% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 95-104% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 95-104% EDTA Plasma (n=4) 99% 95-104%
Sensitivity
< 1.56 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Protein Length
311
NCBI Gene Symbol
APOE
Protein Name
Apolipoprotein E
Gene Name URL
APOE
Nucleotide Accession Number
NM_001305819

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