CA6 ELISA Kit (Human)

CAT: 0247-OKBB01319-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01319-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2 5.3 4.7 6.1 7.4 5.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2 5.3 4.7 6.1 7.4 5.1 CV (%) 6.2 5.3 4.7 6.1 7.4 5.1
CAS Number
7732-18-5
Gene Name
Carbonic anhydrase 6
Gene Aliases
Carbonic anhydrase 6; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase
Gene ID
765
Accession Number
NP_001206.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q18-N308
Target
This gene is mapped to 1p36.23. The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CA6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CA6 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Q18-N308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CA6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CA6
NCBI Gene Symbol
CA6
Protein Name
Carbonic anhydrase 6
Gene Name URL
CA6
Nucleotide Accession Number
NM_001215.3

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