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MANF ELISA Kit (Human)

CAT: 0247-OKBB01261-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01261-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8
CAS Number
7732-18-5
Gene Name
Mesencephalic astrocyte derived neurotrophic factor
Gene Aliases
Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP
Gene ID
7873
Accession Number
NP_006001.4
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: L25-L182
Target
MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human MANF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MANF has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: L25-L182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MANF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
93.6 pg/ml - 6,000 pg/ml
Sensitivity
<15 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human MANF
NCBI Gene Symbol
MANF
Protein Name
Mesencephalic astrocyte-derived neurotrophic factor
Gene Name URL
MANF
Nucleotide Accession Number
NM_006010.5

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