MANF ELISA Kit (Human)

• Reproducibility :

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8 CV (%) 6.2 6.5 4.6 5.1 7.4 6.8

• Gene Name :

  Mesencephalic astrocyte derived neurotrophic factor

• Gene Aliases :

  Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP

• Gene ID :

  7873

• Accession Number :

  NP_006001.4

• Reactivity :

  Human

• Immunogen :

  Expression system for standard: NS0; Immunogen sequence: L25-L182

• Target :

  MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

• Type :

  ELISA Kit

• Applications :

  ELISA

• Assay Principle :

  Aviva's human MANF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MANF has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: L25-L182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MANF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Cell culture supernates, serum and plasma (heparin, EDTA)

• Detection Range :

  93.6 pg/ml - 6,000 pg/ml

• Sensitivity :

  <15 pg/ml

• Reconstitution :

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity :

  Natural and recombinant human MANF

• NCBI Gene Symbol :

  MANF

• Protein Name :

  Mesencephalic astrocyte-derived neurotrophic factor

• Gene Name URL :

  MANF

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_006010.5