MANF Antibody

• Gene Name:

  Mesencephalic astrocyte derived neurotrophic factor

• Gene Aliases:

  Arginine-rich protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET.

• Gene ID:

  7873

• Accession Number:

  NP_006001

• Reactivity:

  Human

• Immunogen:

  Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (25-182AA)

• Target:

  Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311) . Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity) . Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity) . Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057) . Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475) . Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475) . Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057) . Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunohistochemistry

• Purification:

  Affinity Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid

• Reconstitution:

  -20°C or -80°C

• Fragment:

  IgG

• NCBI Gene Symbol:

  MANF

• Host or Source:

  Rabbit

• Protein Name:

  Mesencephalic astrocyte-derived neurotrophic factor

• Gene Name URL:

  MANF

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_006010