CES1 ELISA Kit (Human)

CAT: 0247-OKBB01240-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01240-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 CV (%) 4.2 4.4 5.3 6.5 5.1 7.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 CV (%) 4.2 4.4 5.3 6.5 5.1 7.3 CV (%) 4.2 4.4 5.3 6.5 5.1 7.3
CAS Number
7732-18-5
Gene Name
Carboxylesterase 1
Gene Aliases
Liver carboxylesterase 1; cholesterol acyltransferase; ACAT; Brain carboxylesterase hBr1; Carboxylesterase 1; CE-1; hCE-1; Cocaine carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-acetate deacetylase 1; Monocyte/macrophage serine esterase; Retinyl ester hydrolase; REH; Serine esterase 1; Triacylglycerol hydrolase; TGH; CES1; CES2; SES1
Gene ID
1066
Accession Number
NP_001020365.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: G18-L567
Target
Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CES1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CES1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: G18-L567) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CES1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<0.1 ng/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CES1
NCBI Gene Symbol
CES1
Protein Name
Liver carboxylesterase 1
Gene Name URL
CES1
Nucleotide Accession Number
NM_001025194.1

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