Products for Research Use Only
RegisterLogin

ENPP2 ELISA Kit (Human)

CAT: 0247-OKBB01158-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB01158-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1266 4575 22840 1326 4349 24036 Standard deviation 87.35 297.37 1256.2 92.82 287.03 1562.34 CV (%) 6.9 6.5 5.5 7 6.6 6.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1266 4575 22840 1326 4349 24036 Mean (pg/ml) 1266 4575 22840 1326 4349 24036 Standard deviation 87.35 297.37 1256.2 92.82 287.03 1562.34 Standard deviation 87.35 297.37 1256.2 92.82 287.03 1562.34 CV (%) 6.9 6.5 5.5 7 6.6 6.5 CV (%) 6.9 6.5 5.5 7 6.6 6.5
CAS Number
7732-18-5
Gene Name
Ectonucleotide pyrophosphatase/phosphodiesterase 2
Gene Aliases
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2
Gene ID
5168
Accession Number
NP_001035181.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: A36-I863
Target
Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene. The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Autotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Autotaxin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: A36-I863) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Autotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Autotaxin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin), urine and human milk
Detection Range
625 pg/ml - 40,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human total Autotaxin
NCBI Gene Symbol
ENPP2
Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Gene Name URL
ENPP2
Nucleotide Accession Number
NM_001040092.2

Related Products

CatalogName
OKEH03320-96WENPP2 ELISA Kit (Mouse)
OKEH06955-96WENPP2 ELISA Kit (Rat)
OKCD02408-96WENPP2 ELISA Kit (Rat)
OKAN05938ENPP2 ELISA Kit (Rat) (OKAN05938)
OKCD02408ENPP2 ELISA Kit (Rat) (OKCD02408)
OKEH06955ENPP2 ELISA Kit (Rat) (OKEH06955)
OKEH03320ENPP2 ELISA Kit (Mouse) (OKEH03320)
OACA06797-50ULENPP2 Antibody
OACA06797-100ULENPP2 Antibody
OAEG00958-100UGENPP2 Antibody

Alternative Products

CatalogName
GTR18823625-01Human ENPP2 ELISA Kit
abx378501-01Human Autotaxin (ENPP2) ELISA Development Kit
MBS2704366-01Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit
MBS2578037-01Human ENPP2 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) ELISA Kit
KBH20810GENLISA™ Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA
D1049Ectonucleotide pyrophosphatase/Phosphodiesterase 2 (ENPP2) Human ELISA Kit
32-13687-10ENPP2 Human
abx901729-01Human ENPP2 siRNA
P0551-01Recombinant Human ENPP2
MBS8223955-01ENPP2 siRNA (Human)

Popular Products