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Lgmn ELISA Kit (Mouse)

CAT: 0247-OKBB01092-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01092-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 270 1001 90 264 954 Standard deviation 3.94 12.42 68.06 4.32 15.31 65.82 CV (%) 4.7 4.6 6.8 4.8 5.8 6.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 270 1001 90 264 954 Mean (pg/ml) 84 270 1001 90 264 954 Standard deviation 3.94 12.42 68.06 4.32 15.31 65.82 Standard deviation 3.94 12.42 68.06 4.32 15.31 65.82 CV (%) 4.7 4.6 6.8 4.8 5.8 6.9 CV (%) 4.7 4.6 6.8 4.8 5.8 6.9
CAS Number
7732-18-5
Gene Name
Legumain
Gene Aliases
AEP, Asparaginyl endopeptidase, cysteine 1, Cysteine protease 1, EC 3.4.22.34, Legumain, LGMN, LGMN_HUMAN, LGMN1, Protease, Protease cysteine 1, Protease cysteine 1 (legumain), PRSC1, Q99538
Gene ID
19141
Accession Number
NP_035305.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: V18-Y435
Target
Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1) . Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse Legumain (total) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Legumain (total) has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: V18-Y435) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Legumain (total) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Legumain (total) amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse Legumain (total)
NCBI Gene Symbol
LGMN
Protein Name
Legumain
Gene Name URL
LGMN
Nucleotide Accession Number
NM_011175.3

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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