ORM1 ELISA Kit (Human)

CAT: 0247-OKBB01021-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01021-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1 7.7 4.6 7 9.3 5.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1 7.7 4.6 7 9.3 5.8 CV (%) 6.1 7.7 4.6 7 9.3 5.8
CAS Number
7732-18-5
Gene Name
Orosomucoid 1
Gene Aliases
AGP 1, AGP1, AGP-1, AGP A, AGPA, AGP, OMD 1, ORM, ORM1, Orosomucoid 1, Orosomucoid-1, P02763
Gene ID
5004
Accession Number
NP_000598.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q19-S201
Target
Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human AGP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AGP1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Q19-S201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AGP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human AGP1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<150 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human AGP1
NCBI Gene Symbol
ORM1
Protein Name
Alpha-1-acid glycoprotein 1
Gene Name URL
ORM1
Nucleotide Accession Number
NM_000607.2

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