Products for Research Use Only
RegisterLogin

LOXL2 ELISA Kit (Human)

CAT: 0247-OKBB00953-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00953-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1465 3854 263 1490 4228 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 CV (%) 4.7 7.8 6.6 5.3 8.3 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 252 1465 3854 263 1490 4228 Mean (pg/ml) 252 1465 3854 263 1490 4228 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 CV (%) 4.7 7.8 6.6 5.3 8.3 7.5 CV (%) 4.7 7.8 6.6 5.3 8.3 7.5
CAS Number
7732-18-5
Gene Name
Lysyl oxidase-like 2
Gene Aliases
LOR 2, LOR2, LOX L2, LOXL 2, LOXL2, LOXL2_HUMAN, Lysyl oxidase homolog 2, Lysyl oxidase like 2, Lysyl oxidase like protein 2, Lysyl oxidase related 2, Lysyl oxidase related protein 2, Lysyl oxidase related protein WS9 14, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, WS9 14
Gene ID
4017
Accession Number
NP_002309.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q26-Q774
Target
Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human LOXL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LOXL2 has been precoated onto 96-well plates. Standards (NS0, Q26-Q774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human LOXL2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human LOXL2
NCBI Gene Symbol
LOXL2
Protein Name
Lysyl oxidase homolog 2
Gene Name URL
LOXL2
Nucleotide Accession Number
NM_002318.2

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OKEH08073-96WLOXL2 ELISA Kit (Chicken)
OKEH08072-96WLOXL2 ELISA Kit (Bovine)
OKCD02700-96WLOXL2 ELISA Kit (Mouse)
OKCD02623-96WLOXL2 ELISA Kit (Rat)
OKEH07046-96WLOXL2 ELISA Kit (Mouse)
OKEH07046LOXL2 ELISA Kit (Mouse) (OKEH07046)
OKCD02700LOXL2 ELISA Kit (Mouse) (OKCD02700)
OKAN06453LOXL2 ELISA Kit (Mouse) (OKAN06453)
OKEH08073LOXL2 ELISA Kit (Chicken) (OKEH08073)
OKEH08072LOXL2 ELISA Kit (Bovine) (OKEH08072)

Alternative Products

CatalogName
ELA-E14939hHuman LOXL2 ELISA Kit
E4897-100LOXL2 (Human) ELISA Kit
MBS177562-01Human LOXL2 PicoKine ELISA Kit
GTR18830516-01Human LOXL2 ELISA Kit (Ready to Use)
MBS8118391-01LOXL2 Matched ELISA Antibody Pair Set, Human
JOT-EK5619Hu-01Human Lysyl oxidase homolog 2,LOXL2 ELISA KIT
YP-W13380-01Human Lysyl oxidase homolog 2 (LOXL2) ELISA Kit
E5619Hu-01Human Lysyl oxidase homolog 2, LOXL2 ELISA KIT
EH1679Human LOXL2 (Lysyl oxidase homolog 2) ELISA Kit
E1486HuHuman LOXL2/Lysyl oxidase homolog 2 ELISA Kit

Popular Products