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FGF19 ELISA Kit (Human)

CAT: 0247-OKBB00905-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00905-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 163 386 33 161 403 Standard deviation 2.17 7.82 26.24 2.21 8.37 28.21 CV (%) 6.2 4.8 6.8 6.7 5.2 7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 163 386 33 161 403 Mean (pg/ml) 35 163 386 33 161 403 Standard deviation 2.17 7.82 26.24 2.21 8.37 28.21 Standard deviation 2.17 7.82 26.24 2.21 8.37 28.21 CV (%) 6.2 4.8 6.8 6.7 5.2 7 CV (%) 6.2 4.8 6.8 6.7 5.2 7
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 19
Gene Aliases
FGF-19, FGF19, FGF19_HUMAN, Fibroblast growth factor 19
Gene ID
9965
Accession Number
NP_005108.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: L25-K216
Target
FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human FGF19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF19 has been precoated onto 96-well plates. Standards (E.coli, L25-K216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FGF19 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human FGF19
NCBI Gene Symbol
FGF19
Protein Name
Fibroblast growth factor 19
Gene Name URL
FGF19
Nucleotide Accession Number
NM_005117.2

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