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MPO ELISA Kit (Mouse)

CAT: 0247-OKBB00857-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00857-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 24.97 137.44 346.45 CV (%) 5.4 6.4 5 6.9 8 6.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 24.97 137.44 346.45 Standard deviation 19.11 115.77 266.4 24.97 137.44 346.45 CV (%) 5.4 6.4 5 6.9 8 6.7 CV (%) 5.4 6.4 5 6.9 8 6.7
CAS Number
7732-18-5
Gene Name
Myeloperoxidase
Gene Aliases
84 kDa myeloperoxidase, 89 kDa myeloperoxidase, MPO, MPO Myeloperoxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM_HUMAN
Gene ID
17523
Accession Number
NP_034954.2
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: M16-T718
Target
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse MPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for MPO has been precoated onto 96-well plates. Standards (NS0, M16-T718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse MPO amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse MPO
NCBI Gene Symbol
MPO
Protein Name
Myeloperoxidase
Gene Name URL
MPO
Nucleotide Accession Number
NM_010824.2

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