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CSF1R ELISA Kit (Human)

CAT: 0247-OKBB00821-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00821-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 701 1183 140 669 1090 Standard deviation 5.76 55.37 67.43 6.58 60.21 70.85 CV (%) 4.4 7.9 5.7 4.7 9 6.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131 701 1183 140 669 1090 Mean (pg/ml) 131 701 1183 140 669 1090 Standard deviation 5.76 55.37 67.43 6.58 60.21 70.85 Standard deviation 5.76 55.37 67.43 6.58 60.21 70.85 CV (%) 4.4 7.9 5.7 4.7 9 6.5 CV (%) 4.4 7.9 5.7 4.7 9 6.5
CAS Number
7732-18-5
Gene Name
Colony stimulating factor 1 receptor
Gene Aliases
C FMS, C FMS, CD 115, CD115, CD115 antigen, CFMS, Colony stimulating factor 1 receptor, CSF 1 R, CSF 1R, CSF-1 receptor, CSF-1-R, CSF1 R, CSF1R, CSF1R_HUMAN, CSFR, FIM 2, FIM2, FMS, FMS proto oncogene, FMS protooncogene, HDLS, M-CSF Receptor, M-CSF-R, Macrophage colony stimulating factor 1 receptor, Macrophage colony stimulating factor I receptor, Macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v fms) oncogene homolog, Oncogen FMS, Proto-oncogene c-Fms, V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
Gene ID
1436
Accession Number
NP_001275634.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: I20-E512
Target
CSF1R (Colony stimulating factor 1 receptor), also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CSF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF1R has been precoated onto 96-well plates. Standards (NS0, I20-E512) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CSF1R amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CSF1R
NCBI Gene Symbol
CSF1R
Protein Name
Macrophage colony-stimulating factor 1 receptor
Gene Name URL
CSF1R
Nucleotide Accession Number
NM_001288705.2

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