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NGF ELISA Kit (Monkey)

CAT: 0247-OKBB00727-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00727-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88.1 201.2 469 93.7 231.6 509.2 Standard deviation 7.2 14.5 34.2 7.1 18.8 39.2 CV (%) 6.5 7.2 7.3 7.6 8.1 7.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88.1 201.2 469 93.7 231.6 509.2 Mean (pg/ml) 88.1 201.2 469 93.7 231.6 509.2 Standard deviation 7.2 14.5 34.2 7.1 18.8 39.2 Standard deviation 7.2 14.5 34.2 7.1 18.8 39.2 CV (%) 6.5 7.2 7.3 7.6 8.1 7.7 CV (%) 6.5 7.2 7.3 7.6 8.1 7.7
CAS Number
7732-18-5
Gene Name
Beta-nerve growth factor
Gene Aliases
Beta-nerve growth factor; Beta-NGF; NGF; NGFB
Gene ID
711681
Accession Number
NP_002497.2
Reactivity
Monkey
Immunogen
Expression system for standard: NS0; Immunogen sequence: S122-A241
Target
Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male monkey primate submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is monkey primate 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards (NS0, S122-A241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human NGF amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate NGF/NGF beta
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
NCBI Gene Symbol
NGF
Protein Name
Beta-nerve growth factor
Gene Name URL
NGF
Nucleotide Accession Number
NM_002506.2

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