Progranulin ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 8.6 20 31.5 9.1 25.2 36.8 SD 0.473 1.24 1.638 0.582 1.84 2.392 CV% 5.5 6.2 5.2 6.4 7.3 6.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 8.6 20 31.5 9.1 25.2 36.8 Mean (pg/mL) 8.6 20 31.5 9.1 25.2 36.8 SD 0.473 1.24 1.638 0.582 1.84 2.392 SD 0.473 1.24 1.638 0.582 1.84 2.392 CV% 5.5 6.2 5.2 6.4 7.3 6.5 CV% 5.5 6.2 5.2 6.4 7.3 6.5

• Gene Name:

  Granulin

• Gene Aliases:

  Acrogranin, CLN11, GEP, GP88, Granulin A, Granulin B, Granulin C, Granulin D, Granulin E, Granulin epithelin, Granulin F, Granulin G, Granulin-7, Granulins, GRN, GRN_HUMAN, PC cell derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin

• Gene ID:

  14824

• Accession Number:

  NP_032201

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: T18-L589

• Target:

  Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Progranulin has been precoated onto 96-well plates. Standards (NS0, T18-L589) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Progranulin amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum, and plasma (heparin, EDTA)

• Detection Range:

  0.78 ng/ml - 50 ng/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse Progranulin

• NCBI Gene Symbol:

  GRN

• Protein Name:

  Granulins

• Gene Name URL:

  GRN

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_008175