Products for Research Use Only
RegisterLogin

IL7R/CD127 ELISA Kit (Mouse)

CAT: 0247-OKBB00430-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00430-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 SD 0.114 0.442 0.576 0.145 0.555 0.725 CV% 5.7 6.8 4.5 6.3 7.6 5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 SD 0.114 0.442 0.576 0.145 0.555 0.725 SD 0.114 0.442 0.576 0.145 0.555 0.725 CV% 5.7 6.8 4.5 6.3 7.6 5 CV% 5.7 6.8 4.5 6.3 7.6 5
CAS Number
7732-18-5
Gene Name
Interleukin 7 receptor
Gene Aliases
CD 127, CD127, CD127 antigen, CDw127, IL 7R alpha, IL 7R, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7RA, IL7RA_HUMAN, IL7Ralpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha
Gene ID
16197
Accession Number
NP_032398.3
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: E21-D239
Target
The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7) . The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL7R has been precoated onto 96-well plates. Standards (NS0, E21-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL7R amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants and serum
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse IL7R
NCBI Gene Symbol
IL7R
Protein Name
Interleukin-7 receptor subunit alpha
Gene Name URL
IL7R
Nucleotide Accession Number
NM_008372.4

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OKBB00429IL7R/CD127 ELISA Kit (Human) (OKBB00429)
OKEH02803-96WIL7R ELISA Kit (Human)
OKCD07783-96WIL7R ELISA Kit (Human)
OKCD00851-96WIL7R ELISA Kit (Rat)
OKCD00851IL7R ELISA Kit (Rat) (OKCD00851)
OKCD07783IL7R ELISA Kit (Human) (OKCD07783)
OKEH02803IL7R ELISA Kit (Human) : 96 Wells (OKEH02803)
OPCA05428-100UGIL7R Recombinant Protein (Mouse)
OPCA05428-20UGIL7R Recombinant Protein (Mouse)
OPCA05428-1MGIL7R Recombinant Protein (Mouse)

Alternative Products

CatalogName
EK5295Mouse IL7R/CD127 ELISA Kit
MBS177294-01Mouse IL7R/CD127 PicoKine ELISA Kit
EK0714Mouse IL7R/CD127 ELISA Kit PicoKine®
GR117281Nori® Mouse CD127/IL7R ELISA Kit
EK0714-carrier-freeMouse IL7R/CD127 ELISA Kit PicoKine®, Carrier-free
352941IL7R/CD127, BioAssayTM ELISA Kit (Mouse) (Interleukin 7 Receptor)
E047586Mouse IL7R ELISA Kit
MBS8244622-01Mouse CD127 ELISA Kit
EMS0268Mouse Sil-7R/cd127 ELISA Kit
SEKM-0361Mouse CD127/IL-7RA ELISA Kit

Popular Products