IL7R/CD127 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 SD 0.114 0.442 0.576 0.145 0.555 0.725 CV% 5.7 6.8 4.5 6.3 7.6 5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 Mean (pg/mL) 2 6.5 12.8 2.3 7.3 14.5 SD 0.114 0.442 0.576 0.145 0.555 0.725 SD 0.114 0.442 0.576 0.145 0.555 0.725 CV% 5.7 6.8 4.5 6.3 7.6 5 CV% 5.7 6.8 4.5 6.3 7.6 5

• Gene Name:

  Interleukin 7 receptor

• Gene Aliases:

  CD 127, CD127, CD127 antigen, CDw127, IL 7R alpha, IL 7R, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7RA, IL7RA_HUMAN, IL7Ralpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha

• Gene ID:

  16197

• Accession Number:

  NP_032398.3

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E21-D239

• Target:

  The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7) . The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL7R has been precoated onto 96-well plates. Standards (NS0, E21-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL7R amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants and serum

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse IL7R

• NCBI Gene Symbol:

  IL7R

• Protein Name:

  Interleukin-7 receptor subunit alpha

• Gene Name URL:

  IL7R

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_008372.4