Products for Research Use Only
RegisterLogin

Fetuin A ELISA Kit (Human)

CAT: 0247-OKBB00323-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00323-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 %CV 4.10 5.30 4.79 5.39 6.20 5.69 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 %CV 4.10 5.30 4.79 5.39 6.20 5.69 %CV 4.10 5.30 4.79 5.39 6.20 5.69
CAS Number
7732-18-5
Gene Name
Alpha-2-HS-glycoprotein
Gene Aliases
A2HS, AHS, Ahsg, Alpha 2 HS glycoprotein, Alpha-2-HS-glycoprotein chain B, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, FETUA, FETUA_HUMAN, Fetuin-A, HSGA
Gene ID
197
Accession Number
NP_001613.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: A19-V367
Target
Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Fetuin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fetuin A has been precoated onto 96-well plates. Standards (NS0, A19-V367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fetuin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fetuin A amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum or plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<10 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Notes
Formerly GWB-ZZD177
Specificity
Natural and recombinant human Fetuin A
NCBI Gene Symbol
AHSG
Protein Name
Alpha-2-HS-glycoprotein
Gene Name URL
AHSG
Nucleotide Accession Number
NM_001622.2

Related Products

CatalogName
OKIA00062-96WFetuin ELISA Kit (Human)
OKIA00062Fetuin ELISA Kit (Human) (OKIA00062)
OKWB00296-96WFETUIN A ELISA Kit (Monkey)
OKWB00296FETUIN A ELISA Kit (Monkey) (OKWB00296)
OKRC01156-96WOKRC01156-96W - Fetuin A ELISA Kit (Mouse)
OOSA09715-1GNATIVE FETUIN
OKCD01609-96WFETUB ELISA Kit (Human)
OKCD01609FETUB ELISA Kit (Human) (OKCD01609)
OKBB01028FETUB ELISA Kit (Human) (OKBB01028)
OKCD06003-96WKIT ELISA Kit (Human)

Alternative Products

CatalogName
YP-W18766-01Human Fetuin (Fetuin) ELISA Kit
JL31327-01Human Fetuin B (Fetuin-B) ELISA Kit
YP-W13559-01Human Fetuin-B (Fetuin-B) ELISA Kit
BEK1058Human Fetuin A ELISA Kit
MBS564043-01Human Fetuin ELISA Kit
EK1174-01Human Fetuin-A ELISA Kit
MBS355455-01Fetuin A Human ELISA Kit
E28L0587Human Fetuin-A ELISA Kit
EK710772Human Anti-Fetuin antibody (Fetuin Ab) Elisa Kit
OM619645Bovine Fetuin (Fetuin) ELISA Kit

Popular Products