Fetuin A ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 %CV 4.10 5.30 4.79 5.39 6.20 5.69 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 Mean (ng/mL) 2.17 8.32 12.55 2.39 8.75 13.64 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 St. Dev. 0.089 0.441 0.602 0.129 0.543 0.777 %CV 4.10 5.30 4.79 5.39 6.20 5.69 %CV 4.10 5.30 4.79 5.39 6.20 5.69

• Gene Name:

  Alpha-2-HS-glycoprotein

• Gene Aliases:

  A2HS, AHS, Ahsg, Alpha 2 HS glycoprotein, Alpha-2-HS-glycoprotein chain B, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, FETUA, FETUA_HUMAN, Fetuin-A, HSGA

• Gene ID:

  197

• Accession Number:

  NP_001613.2

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A19-V367

• Target:

  Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human Fetuin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fetuin A has been precoated onto 96-well plates. Standards (NS0, A19-V367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fetuin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fetuin A amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum or plasma (heparin, EDTA)

• Detection Range:

  0.78 ng/ml - 50 ng/ml

• Sensitivity:

  <10 pg/ml

• Homology:

  No detectable cross-reactivity with any other cytokine.

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Notes:

  Formerly GWB-ZZD177

• Specificity:

  Natural and recombinant human Fetuin A

• NCBI Gene Symbol:

  AHSG

• Protein Name:

  Alpha-2-HS-glycoprotein

• Gene Name URL:

  AHSG

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001622.2