BAFF ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 139 312 1508 145 354 1397 St. Dev. 4.9 11.5 58.8 14.4 33.6 124.3 %CV 3.52 3.68 3.89 9.93 9.49 8.89 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 139 312 1508 145 354 1397 Mean (pg/mL) 139 312 1508 145 354 1397 St. Dev. 4.9 11.5 58.8 14.4 33.6 124.3 St. Dev. 4.9 11.5 58.8 14.4 33.6 124.3 %CV 3.52 3.68 3.89 9.93 9.49 8.89 %CV 3.52 3.68 3.89 9.93 9.49 8.89

• Gene Name:

  Tumor necrosis factor (ligand) superfamily, member 13b

• Gene Aliases:

  B lymphocyte stimulator, B-cell-activating factor, BAFF, BLyS, CD257, Dendritic cell-derived TNF-like molecule, soluble form, TALL-1, TN13B_HUMAN, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, Tumor necrosis factor ligand superfamily member 13b

• Gene ID:

  24099

• Accession Number:

  NP_296371.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A127-L309

• Target:

  B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BAFF has been precoated onto 96-well plates. Standards (NS0, A127-L309) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse BAFF amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA)

• Detection Range:

  46.9 pg/ml - 3,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Homology:

  No detectable cross-reactivity with any other cytokine

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse BAFF

• NCBI Gene Symbol:

  TNFSF13B

• Protein Name:

  Tumor necrosis factor ligand superfamily member 13B

• Gene Name URL:

  TNFSF13B

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_033622.1