TNFI ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 126 228 613 135 241 747 St. Dev. 5.42 14.82 35 7.02 16.87 50.05 %CV 4.30 6.5 5.70 5.2 7 6.70 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 126 228 613 135 241 747 Mean (pg/mL) 126 228 613 135 241 747 St. Dev. 5.42 14.82 35 7.02 16.87 50.05 St. Dev. 5.42 14.82 35 7.02 16.87 50.05 %CV 4.30 6.5 5.70 5.2 7 6.70 %CV 4.30 6.5 5.70 5.2 7 6.70

7732-18-5

Tumor necrosis factor

APC1 protein, Cachectin, DIF, soluble form, Tnf, TNF a, TNF alpha, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2, TNF-a, TNF-alpha, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2

21926

NP_001265530.1

Mouse

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2

ELISA Kit

ELISA

Aviva's mouse TNF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from hamster specific for TNF alpha has been precoated onto 96-well plates. Standards (E.coli, L80-L235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TNF alpha amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

15.6 pg/ml - 1,000 pg/ml

<1 pg/ml

No detectable cross-reactivity with any other cytokine.

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant Mouse TNF alpha

TNF

Tumor necrosis factor

TNF