Rantes ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 127 285 498 146 312 587 St. Dev. 6.73 18.24 35.9 12.12 21.22 47.55 %CV 5.29 6.4 7.20 8.30 6.80 8.10 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 127 285 498 146 312 587 Mean (pg/mL) 127 285 498 146 312 587 St. Dev. 6.73 18.24 35.9 12.12 21.22 47.55 St. Dev. 6.73 18.24 35.9 12.12 21.22 47.55 %CV 5.29 6.4 7.20 8.30 6.80 8.10 %CV 5.29 6.4 7.20 8.30 6.80 8.10

• Gene Name:

  Chemokine (C-C motif) ligand 5

• Gene Aliases:

  Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES (4-68), Regulated upon activation normally T expressed and presumably secreted, SCYA 5, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine A5 (RANTES), Small inducible cytokine subfamily A (Cys Cys) member 5, Small-inducible cytokine A5, T cell specific protein p288, T cell specific protein RANTES, T cell specific RANTES protein, T cell-specific protein P228, T-cell-specific protein RANTES, TCP 228, TCP228

• Gene ID:

  20304

• Accession Number:

  NP_038681.2

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: S24-S91

• Target:

  Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Rantes has been precoated onto 96-well plates. Standards (E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Rantes amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA, citrate)

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Homology:

  No detectable cross-reactivity with any other cytokine.

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse Rantes

• NCBI Gene Symbol:

  CCL5

• Protein Name:

  C-C motif chemokine 5

• Gene Name URL:

  CCL5

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_013653.3