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Mouse Rantes ELISA Kit PicoKine®

CAT: 0519-EK0495Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK0495Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
Description
Mouse Rantes ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 32 28 28 29 1.63 5.6% Sample 2 191 178 177 194 185 7.58 4% Sample 3 416 441 428 481 441 24.45 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 32 28 28 29 1.63 5.6% Sample 1 29 32 28 28 29 1.63 5.6% Sample 2 191 178 177 194 185 7.58 4% Sample 2 191 178 177 194 185 7.58 4% Sample 3 416 441 428 481 441 24.45 5.5% Sample 3 416 441 428 481 441 24.45 5.5%
Synonyms
C-C motif chemokine 5; MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES; Ccl5; Scya5
Gene Name
CCL5
Gene ID
20304
UniProt
P30882
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S24-S91
Capture Antibody
Monoclonal antibody from rat
Detection Antibody
Polyclonal antibody from goat
Tissue Specificity
T-cell and macrophage specific.
Applications
ELISA
Field of Research
Antiviral Signaling, Atherosclerosis, Cardiovascular, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Vascular Inflammation
Standard
10 ng/Tube
Sample Type
Cell culture supernatants. serum and plasma (heparin, EDTA, citrate) .
Detection Range
15.6 pg/mL - 1,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 191 416 26 175 442 Standard deviation 1.65 8.97 24.54 1.71 10.85 29.17 CV (%) 5.7% 4.7% 5.9% 6.6% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 191 416 26 175 442 Standard deviation 1.65 8.97 24.54 1.71 10.85 29.17 CV (%) 5.7% 4.7% 5.9% 6.6% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 191 416 26 175 442 Mean (pg/ml) 29 191 416 26 175 442 Standard deviation 1.65 8.97 24.54 1.71 10.85 29.17 Standard deviation 1.65 8.97 24.54 1.71 10.85 29.17 CV (%) 5.7% 4.7% 5.9% 6.6% 6.2% 6.6% CV (%) 5.7% 4.7% 5.9% 6.6% 6.2% 6.6%
Sensitivity
<1 pg/mL
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. .
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
10071 MW
Specificity
Natural and recombinant mouse Rantes
Anticoagulant
Heparin, EDTA or citrate
Gene Name Synonym
C-C motif chemokine 5
Subcellular Location
Secreted.
Protein Name
C-C motif chemokine 5
Sample Data
Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.092 0.175 0.321 0.643 1.101 1.748 2.241 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.092 0.175 0.321 0.643 1.101 1.748 2.241 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.092 0.175 0.321 0.643 1.101 1.748 2.241 O.D. 0.006 0.092 0.175 0.321 0.643 1.101 1.748 2.241
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

UniProtKB · P30882

C-C motif chemokine 5

CCL5_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P30882
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ccl5
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Biological process

ChemotaxisInflammatory response

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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