Leptin ELISA Kit (Human)

CAT: 0247-OKBB00206-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00206-96WSize:96 Well With Removable Strips
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 1026 2535 3542 990 2674 3305 St. Dev. 37.96 81.12 123.97 57.42 120.33 125.59 %CV 3.69 3.2 3.5 5.8 4.5 3.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 1026 2535 3542 990 2674 3305 Mean (pg/mL) 1026 2535 3542 990 2674 3305 St. Dev. 37.96 81.12 123.97 57.42 120.33 125.59 St. Dev. 37.96 81.12 123.97 57.42 120.33 125.59 %CV 3.69 3.2 3.5 5.8 4.5 3.8 %CV 3.69 3.2 3.5 5.8 4.5 3.8
CAS Number
7732-18-5
Gene Name
Leptin
Gene Aliases
FLJ94114, LEP, LEP_HUMAN, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese protein, Obesity factor, Obesity homolog mouse, Obesity murine homolog leptin, OBS
Gene ID
3952
Accession Number
NP_000221.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V22-C167
Target
Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Leptin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Leptin has been precoated onto 96-well plates. Standards (E.coli, V22-C167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Leptin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Homology
No detectable cross-reactivity with any other cytokine
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Notes
Formerly GWB-ZZD030
Specificity
Natural and recombinant human Leptin
NCBI Gene Symbol
LEP
Protein Name
LeptinÂ
Gene Name URL
LEP
Nucleotide Accession Number
NM_000230.2

Related Products

CatalogName
OPPA01996-200UGLeptin Human - Human Leptin Protein
OKRC01444-96WOKRC01444-96W - Leptin ELISA Kit (Rat)
OKRC01079-96WOKRC01079-96W - Leptin ELISA Kit (Rabbit)
OKBB00209Leptin R ELISA Kit (Mouse) (OKBB00209)
OKAG00091-96WOKAG00091-96W - Leptin ELISA Kit (Mouse)
OKRC01238-96WOKRC01238-96W - Leptin ELISA Kit (Mouse)
OPCA00669-20UGRecombinant human Leptin
OPCA00669-1MGRecombinant human Leptin
OPCA00669-100UGRecombinant human Leptin
OKRC01239-96WOKRC01239-96W - Leptin R ELISA Kit (Mouse)

Alternative Products

CatalogName
EK710057Human Leptin (Leptin) Elisa Kit
EA-0202Human Leptin ELISA
EKF57080Human Leptin R (Leptin receptor) ELISA Kit
EKA51863Human Leptin ELISA Kit
E6273Leptin) Human ELISA Kit
K4777-100Leptin (human) ELISA Kit
PT_41403-01Human Leptin (Leptin Human)
MD53001Leptin ELISA
EK765057Camel Leptin (Leptin) Elisa Kit

Popular Products