Activin A ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 95 289 453 98 301 495 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 %CV 5.05 5.19 5.20 7.95 6.21 4.90 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 95 289 453 98 301 495 Mean (pg/mL) 95 289 453 98 301 495 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 %CV 5.05 5.19 5.20 7.95 6.21 4.90 %CV 5.05 5.19 5.20 7.95 6.21 4.90

7732-18-5

Inhibin, beta A

Activin beta A chain, erythroid differentiation factor, erythroid differentiation protein, follicle stimulating hormone releasing protein, FSH releasing protein, Inhibin, inhibin beta A chain

16323

NP_032406.1

Mouse

Expression system for standard: CHO; Immunogen sequence: G311-S426

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

ELISA Kit

ELISA

Aviva's mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards (CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Activin A amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernates, serum, plasma (heparin, EDTA) and saliva

15.6 pg/ml - 1,000 pg/ml

<10 pg/ml

No detectable cross-reactivity with any other cytokine.

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant mouse Activin A

INHBA

Inhibin beta A chain

INHBA