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Activin A ELISA Kit (Mouse)

CAT: 0247-OKBB00124-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00124-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 95 289 453 98 301 495 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 %CV 5.05 5.19 5.20 7.95 6.21 4.90 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 95 289 453 98 301 495 Mean (pg/mL) 95 289 453 98 301 495 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 St. Dev. 4.8 15 23.6 7.8 18.7 24.3 %CV 5.05 5.19 5.20 7.95 6.21 4.90 %CV 5.05 5.19 5.20 7.95 6.21 4.90
CAS Number
7732-18-5
Gene Name
Inhibin, beta A
Gene Aliases
Activin beta A chain, erythroid differentiation factor, erythroid differentiation protein, follicle stimulating hormone releasing protein, FSH releasing protein, Inhibin, inhibin beta A chain
Gene ID
16323
Accession Number
NP_032406.1
Reactivity
Mouse
Immunogen
Expression system for standard: CHO; Immunogen sequence: G311-S426
Target
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards (CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Activin A amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse Activin A
NCBI Gene Symbol
INHBA
Protein Name
Inhibin beta A chain
Gene Name URL
INHBA
Nucleotide Accession Number
NM_008380.1

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