Creatinine Serum Kit

• Reproducibility:

  Sample Creatinine Conc. (mg/dL) %CV 1 0.99 7.9 2 1.50 6.3 3 3.82 4.5 Sample Creatinine Conc. (mg/dL) %CV Sample Creatinine Conc. (mg/dL) %CV 1 0.99 7.9 1 0.99 7.9 2 1.50 6.3 2 1.50 6.3 3 3.82 4.5 3 3.82 4.5 Sample Creatinine Conc. (mg/dL) %CV 1 0.91 9.6 2 1.26 7.3 3 3.51 8.0 Sample Creatinine Conc. (mg/dL) %CV Sample Creatinine Conc. (mg/dL) %CV 1 0.91 9.6 1 0.91 9.6 2 1.26 7.3 2 1.26 7.3 3 3.51 8.0 3 3.51 8.0

• Gene Name:

  Creatinine

• Type:

  Application Kit

• Assay Principle:

  The DetectX® Serum Creatinine Kits are designed to quantitatively measure creatinine present in serum samples. Please read the complete kit insert before performing this assay. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the DetectX® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Mammalian Serum and Plasma

• Sensitivity:

  0.081 mg/dL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear Half-Area 96 well Plates 2 or 4 each Creatinine Standard 100 uL or 1 mL Assay Diluent 6 mL or 12 mL DetectX® Creatinine Reagent 20 mL or 50 mL Component Quantity Component Quantity Clear Half-Area 96 well Plates 2 or 4 each Clear Half-Area 96 well Plates 2 or 4 each Creatinine Standard 100 uL or 1 mL Creatinine Standard 100 uL or 1 mL Assay Diluent 6 mL or 12 mL Assay Diluent 6 mL or 12 mL DetectX® Creatinine Reagent 20 mL or 50 mL DetectX® Creatinine Reagent 20 mL or 50 mL