8-OHdG ELISA Kit

Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV 1 423.6 11.7 2 995.7 8.2 3 1,187 7.1 Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV 1 423.6 11.7 1 423.6 11.7 2 995.7 8.2 2 995.7 8.2 3 1,187 7.1 3 1,187 7.1 Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV 1 404.9 13.4 2 887.8 8.3 3 1,121 8.1 Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV Sample 8-Hydroxy-2'-deoxyguanosine Conc. (pg/mL) %CV 1 404.9 13.4 1 404.9 13.4 2 887.8 8.3 2 887.8 8.3 3 1,121 8.1 3 1,121 8.1

7732-18-5

ELISA Kit

Enzyme-linked immunosorbent assay

The DetectX® DNA Damage Immunoassay Kit is designed to quantitatively measure DNA and RNA oxidized guanosine species. The assay detects all three oxidized guanine species; 8-hydroxy-2’-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA and 8-hydroxyguanine, from digested DNA from DNA or RNA. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. An 8-hydroxy-2’-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2’-deoxyguanosine to each well. After a 2 hour incubation, the plate is washed and substrate added. The substrate reacts with the peroxidaselabeled antibody that has reacted with the bound conjugate. After the reaction is stopped, the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the 8-hydroxy-2’-deoxyguanosine in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, EDTA and Heparin Plasma, Saliva, Urine, Digested DNA, Fecal Extracts and Tissue Culture Media

50.9 pg/mL

2°C to 8°C

Component Quantity Coated Clear 96 Well Plate 1 or 5 Each 8-Hydroxy-2'-deoxyguanosine Standard 70 uL or 350 uL DetectX® 8-Hydroxy-2'-deoxyguanosine Antibody 3 mL or 13 mL DetectX® 8-Hydroxyguanosine EIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plate 1 or 5 Each Coated Clear 96 Well Plate 1 or 5 Each 8-Hydroxy-2'-deoxyguanosine Standard 70 uL or 350 uL 8-Hydroxy-2'-deoxyguanosine Standard 70 uL or 350 uL DetectX® 8-Hydroxy-2'-deoxyguanosine Antibody 3 mL or 13 mL DetectX® 8-Hydroxy-2'-deoxyguanosine Antibody 3 mL or 13 mL DetectX® 8-Hydroxyguanosine EIA Conjugate 3 mL or 13 mL DetectX® 8-Hydroxyguanosine EIA Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each

8-OHDG