17-Hydroxyprogesterone ELISA Kit

Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV 1 1,265.7 5.4 2 450.0 6.5 3 168.5 7.9 Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV 1 1,265.7 5.4 1 1,265.7 5.4 2 450.0 6.5 2 450.0 6.5 3 168.5 7.9 3 168.5 7.9 Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV 1 1,204.8 7.0 2 444.1 6.5 3 162.9 10.6 Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV Sample 17-Hydroxyprogesterone Conc. (pg/mL) %CV 1 1,204.8 7.0 1 1,204.8 7.0 2 444.1 6.5 2 444.1 6.5 3 162.9 10.6 3 162.9 10.6

7732-18-5

17-Hydroxyprogesterone

ELISA Kit

Enzyme-linked immunosorbent assay

The DetectX® 17-Hydroxyprogesterone Immunoassay kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. The kit will quantitatively measure 17HO-P present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the 17HOP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dried Fecal Extracts, Urine, Extracted Serum/Plasma, and Tissue Culture Media

20.3 pg/mL

2°C to 8°C