Creatinine Urinary Detection Kit

• Reproducibility:

  Sample Creatinine Conc. (mg/dL) %CV 1 8.92 2.8 2 4.08 1.3 3 1.94 2.5 4 1.11 3.0 Sample Creatinine Conc. (mg/dL) %CV Sample Creatinine Conc. (mg/dL) %CV 1 8.92 2.8 1 8.92 2.8 2 4.08 1.3 2 4.08 1.3 3 1.94 2.5 3 1.94 2.5 4 1.11 3.0 4 1.11 3.0 Sample Creatinine Conc. (mg/dL) %CV 1 9.04 2.3 2 4.18 2.7 3 2.03 3.9 4 1.18 3.7 Sample Creatinine Conc. (mg/dL) %CV Sample Creatinine Conc. (mg/dL) %CV 1 9.04 2.3 1 9.04 2.3 2 4.18 2.7 2 4.18 2.7 3 2.03 3.9 3 2.03 3.9 4 1.18 3.7 4 1.18 3.7

• Gene Name:

  Creatinine Urinary

• Type:

  Application Kit

• Assay Principle:

  The DetectX® Urinary Creatinine Kit is designed to quantitatively measure creatinine present in urine samples. Please read the complete kit insert before performing this assay. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the DetectX® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Human, Monkey, Dog, and Rat Urine

• Sensitivity:

  0.019 mg/dL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear 96 Well Plates 2 plates,2 by 5 plates Creatinine Standard 1 mL,2 by 1 mL DetectX® Creatinine Reagent 20 mL,2 by 50 mL Plate Sealers 2 each,10 each Component Quantity Component Quantity Clear 96 Well Plates 2 plates,2 by 5 plates Clear 96 Well Plates 2 plates,2 by 5 plates Creatinine Standard 1 mL,2 by 1 mL Creatinine Standard 1 mL,2 by 1 mL DetectX® Creatinine Reagent 20 mL,2 by 50 mL DetectX® Creatinine Reagent 20 mL,2 by 50 mL Plate Sealers 2 each,10 each Plate Sealers 2 each,10 each