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NIH/3T3 Whole Cell Lysate

CAT: 0247-OCRA00122-500UGSize: 500 µgDry Ice: NoHazardous: No
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CAT#:0247-OCRA00122-500UGSize:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Reactivity
Mouse
Conjugation
Unconjugated
Type
Cell Lysate
Applications
Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
Varies by lot. See vial for concentration.
Format
Liquid// 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) with 10% (v/v) glycerol
Reconstitution
Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles. Expiration date is three (3) months from date of opening if stored at -20° C or (1) year from date of opening if stored at -70° C.

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Alternative Products

CatalogName
sc-2210NIH/3T3 Whole Cell Lysate
LYSATE0039Mouse NIH/3T3 Whole Cell Lysate
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LO00016VNIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell Lysate
sc-24794NIH/3T3 (IP) Cell Lysate
sc-2247NIH/3T3 + anisomycin Cell Lysate
sc-24752Quiescent NIH/3T3 Cell Lysate
sc-3804NIH/3T3 + UV Cell Lysate
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