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EIF2AK2 Antibody (Phospho-Thr451)

CAT: 0247-OASG05951-100ULSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0247-OASG05951-100ULSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Eukaryotic translation initiation factor 2-alpha kinase 2
Gene Aliases
PKR, PRKR, LEUDEN, EIF2AK1, PPP1R83
Gene ID
5610
Reactivity
Human
Immunogen
Synthesized peptide derived from human PKR around the phosphorylation site of T451. Location: 390-470aa.
Target
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
IHC
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Molecular Weight
70 kDa
Fragment
IgG
Specificity
Phospho-PKR (T451) Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at T451.
NCBI Gene Symbol
EIF2AK2
Host or Source
Rabbit
Gene Name URL
EIF2AK2

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