EIF2AK2 Antibody (Phospho-Thr258) : HRP (OAAF00474-HRP)

• Gene Name:

  Interferon-induced, double-stranded RNA-activated protein kinase

• Gene Aliases:

  PKR, PRKR, LEUDEN, EIF2AK1, PPP1R83

• Gene ID:

  5610

• Swiss Prot:

  P19525

• Accession Number:

  NP_001129123.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived between 226-275 amino acids from the Internal region of human PKR around the phosphorylation site of Thr258.

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, ELISA

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  0.6-0.7 mg/mL

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Specificity:

  PKR (Phospho-Thr258) antibody detects endogenous levels of PKR only when phosphorylated at threonine 258.

• Applications Notes:

  WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000

• NCBI Gene Symbol:

  EIF2AK2

• Protein Name:

  Interferon-induced, double-stranded RNA-activated protein kinase