GAPDH Antibody

• Gene Name:

  Glyceraldehyde-3-phosphate dehydrogenase

• Gene Aliases:

  G3PD, GAPD, HEL-S-162eP

• Gene ID:

  2597; 100042025; 24383

• Reactivity:

  Human, Mouse, Rat, Monkey, Dog, Cow, Hamster, Rabbit, Pig, Sheep

• Immunogen:

  Synthetic Peptide

• Target:

  This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Applications:

  WB

• Purification:

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid. 1 mg/mL in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Specificity:

  The antibody detects endogenous GAPDH protein.

• NCBI Gene Symbol:

  GAPDH, GAPDH-PS15

• Host or Source:

  Mouse

• Gene Name URL:

  GAPDHGAPDH-PS15

• CAS Number:

  9007-83-4