Anti-Syntaxin 1A

• Gene Aliases:

  STX1, HPC-1, P35-1, SYN1A

• Gene ID:

  6804

• Accession Number:

  NP_001159375.1

• Reactivity:

  Human, Rat, Pig

• Immunogen:

  Synaptic immunoprecipitate (crude) from human brain.

• Target:

  Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007) . Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010) .

• Clonality:

  Monoclonal

• Clone:

  SP8

• Type:

  Monoclonal Antibody

• Applications:

  WB, IHC

• Purification:

  Protein G purified culture supernatant.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Homology:

  Human; Pig; Rat

• Format:

  Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

• Reconstitution:

  Store at -20C; stable for at least one year.

• Molecular Weight:

  ~36kDa

• Fragment:

  1

• Specificity:

  The antibody is specific for the ~ 36 kDa syntaxin protein in Western blots of rat brain lysate. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. Does not work on paraffin sections.

• NCBI Gene Symbol:

  STX1A

• Host or Source:

  Mouse

• Protein Name:

  Syntaxin-1A

• Gene Name URL:

  STX1A

• CAS Number:

  9007-83-4