Anti-Peripherin

• Gene ID:

  3414

• Reactivity:

  Most mammals

• Immunogen:

  Recombinant rat peripherin expressed in and purified from E. coli.

• Target:

  Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1) . Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2) . Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3) . Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

• Clonality:

  Monoclonal

• Clone:

  7C5

• Type:

  Monoclonal Antibody

• Applications:

  WB, IF

• Purification:

  Unpurified, concentrated culture supernatant.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Homology:

  Most mammals

• Format:

  Liquid.

• Reconstitution:

  Store at -207deg; C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

• Molecular Weight:

  ~57kDa

• Fragment:

  1

• Specificity:

  Specific for the ~57kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.

• NCBI Gene Symbol:

  PRPH

• Host or Source:

  Mouse

• Protein Name:

  Peripherin

• Gene Name URL:

  PRPH

• CAS Number:

  9007-83-4