Anti-Actin

• Gene ID:

  421534

• Accession Number:

  NP_001026234.1

• Reactivity:

  Zebrafish

• Immunogen:

  Chicken gizzard actin.

• Target:

  Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

• Clonality:

  Monoclonal

• Clone:

  C4

• Type:

  Monoclonal Antibody

• Applications:

  WB, IF, IHC

• Purification:

  Unpurified ascites fluid.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Homology:

  Zebrafish

• Format:

  Liquid containing 0.01% sodium azide.

• Reconstitution:

  Store at -20C in undiluted aliquots; stable for at least one year. Avoid freeze/thaw cycles.

• Molecular Weight:

  ~42kDa

• Fragment:

  1

• Specificity:

  Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.

• NCBI Gene Symbol:

  ACTA1

• Host or Source:

  Mouse

• Protein Name:

  Actin, alpha skeletal muscle

• Gene Name URL:

  ACTA1

• CAS Number:

  9007-83-4