UBE2N Antibody

• Gene Name:

  Ubiquitin conjugating enzyme E2 N

• Gene Aliases:

  Bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; HEL-S-71; UBC13; UbcH13; UbcH-ben; UBCHBEN; UBC13; ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13) ; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) ; ubiquitin-protein ligase N; yeast UBC13 homolog.

• Gene ID:

  7334

• Accession Number:

  https://www.ncbi.nlm.nih.gov/protein/AAP35519

• Reactivity:

  Bovine|Human|Mouse|Rat

• Immunogen:

  UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13. The immunogen is located within the last 50 amino acids of UBC13.

• Target:

  The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot

• Purification:

  Ion Exchange

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Human; Mouse; Rat

• Format:

  Liquid. UBC13 Antibody is supplied in PBS containing 0.02% sodium azide.

• Reconstitution:

  UBC13 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Notes:

  Formerly GWB-5215C8

• NCBI Gene Symbol:

  UBE2N

• Host or Source:

  Rabbit

• Protein Name:

  Ubiquitin-conjugating enzyme E2 N|ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) [Homo sapiens]

• Gene Name URL:

  UBE2N

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_003348.3