CDKN1A Antibody

• Gene Name:

  Cyclin dependent kinase inhibitor 1A

• Gene Aliases:

  P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1

• Gene ID:

  1026

• Accession Number:

  NP_000380.1

• Reactivity:

  Human

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

• Target:

  This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

• Clonality:

  Monoclonal

• Clone:

  GT8611

• Type:

  Monoclonal Antibody

• Applications:

  ICC, IF, WB

• Purification:

  Affinity purified by Protein G.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid

• Buffer:

  PBS

• Reconstitution:

  Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

• Fragment:

  IgG2b

• NCBI Gene Symbol:

  CDKN1A

• Host or Source:

  Mouse

• Protein Name:

  Cyclin-dependent kinase inhibitor 1

• Gene Name URL:

  CDKN1A

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000389.4