PFKL antibody

• Gene Name:

  6-phosphofructokinase, liver type

• Gene Aliases:

  PFK-B, PFK-L, ATP-PFK

• Gene ID:

  5211

• Accession Number:

  NP_002617.3

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

• Target:

  Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L) . PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  ICC, IF, IHC-FFPE, WB

• Purification:

  Purified by antigen-affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  Varies by lot. See vial for concentration.

• Format:

  Liquid// 0.1M Tris-Glycine (pH 7) with 10% glycerol and 0.01% Thimerosal

• Buffer:

  0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

• Reconstitution:

  Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze/thaw cycles.

• Molecular Weight:

  85 kDa

• Fragment:

  IgG

• NCBI Gene Symbol:

  PFKL

• Host or Source:

  Rabbit

• Protein Name:

  6-phosphofructokinase, liver type

• Gene Name URL:

  PFKL

• CAS Number:

  9007-83-4