CD28 Antibody

• Gene Name:

  CD28 molecule

• Gene Aliases:

  T-cell-specific surface glycoprotein CD28; Tp44.

• Gene ID:

  940

• Accession Number:

  NP_001230006.1NP_001230007.1NP_006130.1

• Reactivity:

  Human|Non-human Primate

• Immunogen:

  DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

• Target:

  Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933) . Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037) .

• Clonality:

  Monoclonal

• Clone:

  CD28.2

• Conjugation:

  FITC

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry

• Purification:

  Purified by size-exclusion chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  20 ul / test

• Format:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG1

• Specificity:

  HLDA V.; WS Code 5T CD28.05//

• NCBI Gene Symbol:

  CD28

• Host or Source:

  Mouse

• Protein Name:

  T-cell-specific surface glycoprotein CD28

• Gene Name URL:

  CD28

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001243077.1NM_001243078.1NM_006139.3