LIME1 Antibody

• Gene Name:

  Lck interacting transmembrane adaptor 1

• Gene Aliases:

  DJ583P15.4; lck-interacting membrane protein; lck-interacting molecule; lck-interacting transmembrane adapter 1; LIME.

• Gene ID:

  54923

• Accession Number:

  NP_060276.2

• Reactivity:

  Human

• Immunogen:

  Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

• Target:

  Involved in BCR (B-cell antigen receptor) -mediated signaling in B-cells and TCR (T-cell antigen receptor) -mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.

• Clonality:

  Monoclonal

• Clone:

  LIME-06

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry|Immunoprecipitation

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG1

• Specificity:

  The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

• NCBI Gene Symbol:

  LIME1

• Host or Source:

  Mouse

• Protein Name:

  Lck-interacting transmembrane adapter 1

• Gene Name URL:

  LIME1

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_017806.3