LIMK1 Antibody

CAT: 0247-OAAF00663-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0247-OAAF00663-100UGSize:100 µg
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CAS Number
9007-83-4
Gene Name
LIM domain kinase 1|LIM domain kinase 2
Gene Aliases
LIM domain kinase 1; LIM domain kinase 2; LIM motif-containing protein kinase; LIMK; LIMK-1.
Gene ID
3984|3985
Swiss Prot
P53667|P53671
Accession Number
NP_001026971.1
Reactivity
Human|Monkey|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human LIMK1/2.
Target
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.|Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
72 kDa
Notes
Formerly GWB-ASC071
Specificity
LIMK1/2 (Ab-508/505) antibody detects endogenous levels of total LIMK1/2 protein.
NCBI Gene Symbol
LIMK1|LIMK2
Host or Source
Rabbit
Protein Name
LIM domain kinase 1|LIM domain kinase 2
Gene Name URL
LIMK1|LIMK2

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