CD14 antibody

• Gene Name:

  CD14 molecule

• Gene Aliases:

  Monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein.

• Gene ID:

  929

• Accession Number:

  NP_000582.1NP_001035110.1NP_001167575.1NP_001167576.1

• Reactivity:

  Human|Non-human Primate

• Immunogen:

  A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

• Target:

  Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655) . In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135) . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211) . Binds electronegative LDL (LDL (-) ) and mediates the cytokine release induced by LDL (-) (PubMed:23880187) .

• Clonality:

  Monoclonal

• Clone:

  MEM-18

• Type:

  Monoclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Flow cytometry|Functional assay|Immunoprecipitation|Western blot

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4

• Buffer:

  Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG1

• Specificity:

  HLDA III; WS Code M 253 HLDA IV; WS Code M 314 HLDA V; WS Code M MA087 HLDA VI; WS Code M MA95//

• NCBI Gene Symbol:

  CD14

• Host or Source:

  Mouse

• Protein Name:

  Monocyte differentiation antigen CD14

• Gene Name URL:

  CD14

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000591.3NM_001040021.2NM_001174104.1NM_001174105.1