CD14 Protein

• Gene Aliases:

  Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, CD14 Human, CHO, CD14 Human Recombinant, CHO

• Gene ID:

  929

• Accession Number:

  NP_000582.1

• Reactivity:

  Human

• Target:

  CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

• Type:

  Protein

• Source:

  CHO Cells

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Bioactivity:

  Up to 20 ug/ml CD14 inhibit binding of FITC-LPS (0.5 ug/ml) to 600,000 CD14+CHO transfectants (FACS) .

• Format:

  CD14 was lyophilized from a concentrated protein solution (1.0 mg/ml) containing phosphate-buffered saline, pH 7.2. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  Reconstitution: //It is recommended to reconstitute the lyophilized CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml. Further dilutions should be made with phosphate buffered saline (PBS) . Storage: //Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD14 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA) . Please prevent freeze-thaw cycles.Storage: //Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD14 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Notes:

  Formerly GWB-A30559

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  CD14-CHO

• Protein Name:

  Monocyte differentiation antigen CD14

• Gene Name URL:

  CD14-CHO