ENG Antibody

• Gene Name:

  Endoglin

• Gene Aliases:

  CD105 antigen; END; endoglin; HHT1; ORW1.

• Gene ID:

  2022

• Accession Number:

  NP_000109.1NP_001108225.1NP_001265067.1

• Reactivity:

  Human|Porcine

• Immunogen:

  Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

• Target:

  Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529) . Required for normal structure and integrity of adult vasculature (PubMed:7894484) . Regulates the migration of vascular endothelial cells (PubMed:17540773) . Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity) . May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity) . May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830) . Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:8370410, PubMed:21737454, PubMed:22347366, PubMed:23300529) . Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529) .

• Clonality:

  Monoclonal

• Clone:

  MEM-229

• Type:

  Monoclonal Antibody

• Applications:

  Flow cytometry|Immunocytochemistry|Immunohistochemistry-Frozen|Western blot

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer:

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution:

  2°C to 8°C

• Fragment:

  IgG2a

• Specificity:

  The antibody MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

• NCBI Gene Symbol:

  ENG

• Host or Source:

  Mouse

• Protein Name:

  Endoglin

• Gene Name URL:

  ENG

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000118.3NM_001114753.2NM_001278138.1