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NLRP3 Antibody

CAT: 0247-OAED00356-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAED00356-100UGSize:100 µg
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CAS Number
9007-83-4
Gene Name
NLR family pyrin domain containing 3
Gene Aliases
AGTAVPRL; AII; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; caterpiller protein 1.1; CIAS1; CLR1.1; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1.
Gene ID
114548
Accession Number
NP_001073289.1NP_001120933.1NP_001120934.1NP_001230062.1NP_004886.3NP_899632.1
Reactivity
Human
Immunogen
Recombinant human NLRP3/NALP3 (NACHT-, LRR- and PYD-containing protein 3)  (pyrin domain) .
Target
As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5) . Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494) . The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K (+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (PubMed:23871209) . Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity) . During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity) .
Clonality
Monoclonal
Clone
Nalpy3-b
Type
Monoclonal Antibody
Source
Purified from concentrated hybridoma tissue culture supernatant.
Applications
Immunohistochemistry|Immunoprecipitation|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1mg/ml
Reconstitution
Long Term Storage : //-20C Short Term Storage : //+4C Handling : //Avoid freeze/thaw cycles. Short Term Storage : //+4C Handling : //Avoid freeze/thaw cycles. Handling : //Avoid freeze/thaw cycles.
Fragment
IgG1
Specificity
Recognizes human NLRP3/NALP3. Detects endogenous protein by IP and WB.
NCBI Gene Symbol
NLRP3
Host or Source
Mouse
Protein Name
NACHT, LRR and PYD domains-containing protein 3
Gene Name URL
NLRP3
Nucleotide Accession Number
NM_001079821.2NM_001127461.2NM_001127462.2NM_001243133.1NM_004895.4NM_183395.2

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