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HSP104 Antibody

CAT: 0247-OAED00132-50UGSize: 50 µgDry Ice: NoHazardous: No
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CAT#:0247-OAED00132-50UGSize:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Chaperone ATPase HSP104
Gene Aliases
Chaperone ATPase HSP104; Protein aggregation-remodeling factor HSP104; YLL026W.
Gene ID
850633
Accession Number
NP_013074.1
Reactivity
Yeast
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of yeast Hsp104.
Target
Required, in concert with Hsp40 (YDJ1) and Hsp70 (SSA1) and small Hsps (HSP26), for the dissociation, resolubilization and refolding of aggregates of damaged proteins after heat or other environmental stresses. Extracts proteins from aggregates by unfolding and threading them in an ATP-dependent process through the axial channel of the protein hexamer, after which they can be refolded by components of the Hsp70/Hsp40 chaperone system. Substrate binding is ATP-dependent, and release of bound polypeptide is triggered by ATP hydrolysis. Also responsible for the maintenance of prions by dissociating prion fibrils into smaller oligomers, thereby producing transmissible seeds that can infect daughter cells during mitosis and meiosis. Loss of HSP104 can cure yeast cells of the prions [PSI+], [URE3] and [PIN+]. Excess HSP104 can also specifically cure cells of [PSI+].
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Western blot
Purification
Protein A affinity purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid.// PBS containing 50% glycerol and 0.09% sodium azide.
Reconstitution
Store long-term at -20C. Avoid freeze/thaw cycles.
Molecular Weight
104 kDa
Specificity
Recognizes yeast HSP104. Detects a band of ~104kDa by Western blot.
NCBI Gene Symbol
HSP104
Host or Source
Rabbit
Protein Name
Heat shock protein 104
Gene Name URL
HSP104
Nucleotide Accession Number
NM_001181846.1

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