CD95 Antibody

• Gene Name:

  Fas cell surface death receptor

• Gene Aliases:

  APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6

• Gene ID:

  355

• Accession Number:

  NP_000034.1

• Reactivity:

  Human

• Target:

  The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD) . The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

• Clonality:

  Monoclonal

• Clone:

  B-D29

• Type:

  Monoclonal Antibody

• Applications:

  FC

• Purification:

  Ion exchange chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 ug/uL

• Bioactivity:

  Inhibits FasL induced apoptosis

• Format:

  Liquid

• Buffer:

  Phosphate-buffered saline. Sterile-filtered through 0.22 um and treated to remove endotoxins. Carrier and preservative free

• Reconstitution:

  Stable at +2-8C for 12 months. For longer storage, freeze aliquots

• Molecular Weight:

  38 kDa

• Fragment:

  Mouse IgG1

• Specificity:

  Recognises the Fas, APO1 antigen, a 45 kDa protein

• NCBI Gene Symbol:

  CD95

• Host or Source:

  Human

• Protein Name:

  Tumor necrosis factor receptor superfamily member 6

• Gene Name URL:

  CD95

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000043.4